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KMID : 1007520020110050484
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Food Science and Biotechnology
2002 Volume.11 No. 5 p.484 ~ p.489
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Antioxidative and Antimicrobial Effects of Glycoprotein Isolated from Solanum nigrum Linne
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Lim, Kye Taek
Heo, Kyung Sun/Son, Young Ok
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Abstract
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The antioxidative effects and antimicrobial effects of glycoprotein from Solanum nigrum Linne (SNL glycoprotein) was tested with hydroxyl radical (OH)-exposed NIH/3T3 and microorganisms such as Escherichia coli (JA221, XL1-Blue), and Listeria monocytogenes. It was detected one glycoprotein (150 kDa, SNL glycoprotein Ⅰ) from SNL fruits and the other (210 kDa, SNL glycoprotein Ⅱ) from SNL stems and leaves on 10% SDS-PAGE, and used for further experiment. From the antioxidative test, both glycoprotein Ⅰ and glycoprotein Ⅱ were revealed to have protective effects against hydroxyl radical (OH) at 10㎍/mL (p<0.05) and at 20㎍/mL (p<0.01), respectively. The growths of JA221 and XL1-Blue were inhibited up to 25, and 41 % by the addition of 1㎍/mL SNL glycoprotein Ⅰ, while 28, and 52% at the same concentration of SNL glycoprotein Ⅱ, respectively. In Listeria monocytogenes, the growth inhibition was 28, and 35% at 0.5㎍/mL SNL glycoprotein Ⅰ and SNL glycoprotein Ⅱ, respectively, and both glycoproteins have similar effects at 1㎍/mL. From our results, it was concluded that SNL glycoprotein Ⅰ and Ⅱ have scavenging activities on hydroxyl radicals and inhibitory effects on microorganisms.
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